The WesternC standards are shown below on a PVDF membrane after a transfer using the Trans-Blot ® Turbo™ transfer system, which enables protein transfers in as little as three minutes. Additionally, the dual-color feature of the standard provides a quick way to look at the orientation of your blot. WesternC protein standards also enable easy monitoring of transfer process, providing a broad molecular weight range, from 10 to 250 kD. ![]() Once the ten standard bands are separated and visualized, you can move onto the next step of the workflow. The WesternC standards are shown below after electrophoretic separation on a 4–20% Mini-PROTEAN ® TGX™ gel, which allows protein separation in as little as 15 minutes. WesternC protein standards have dual-colored prestained bands that enable you to monitor electrophoretic separation. Monitor Your Western Blotting Workflow with Precision Plus Protein WesternC standards The WesternC standards have dyes that fluoresce under green and red wavelengths. *StrepTactin-HRP or StrepTactin-AP conjugate is needed for colorimetric and chemiluminescent blot development, but not for fluorescence development. The following workflow illustrates the use of Precision Plus Protein WesternC standard with a suite of Bio-Rad products, all of which minimize the time to results for your western blotting workflow. This protein standard can be used regardless of your detection method* (colorimetric, chemiluminescent or fluorescent). This protein standard is unique in that it offers the ability to visualize and monitor electrophoretic separation, assess transfer of proteins to your membrane, and visualize and estimate the molecular weight of your target after blot development with a StrepTactin-HRP or AP conjugate. ![]() Bio-Rad’s Precision Plus Protein WesternC standard enables you to check the progression and success of all the major steps in the western blotting process. ![]() Thus, having a tool to monitor each of the major steps in the western blotting workflow reduces the chances of finding out at the very end of the workflow that an experiment was not successful. The whole western blotting workflow can take up to three days to complete. Western blotting, although a commonly used technique, involves a time-consuming multi step process.
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